Hi, my name is Dominik Lenk and I am a biostatistician at Stegmann Systems. In this article I will show you how to map an actual plate design to a Quantitative Response Assay document in PLA 3. Therefore i will set up a 96 well plate with 1 standard sample and 3 test samples. If you are not familiar with the configuration of assay elements and preparation schemes, i recommend Andreas Fromkorth's blog article "My first Assay".
Definition of the plate size
The first step on the way to the plate design is the definition of the plate size. Therefore I open the 'Content' view of the document and add two position factor columns to my observation data. One of these factors contains information about the rows, the other one contains information about the columns of the plate. The actually given identifiers (via the property 'Name') will end up as column names in the 'Observation' view of the document. For my example, i use the obvious names 'Row' and 'Column'. I set the property 'Number of Positions' to 8 for 'Row' and to 12 for 'Column'. As I want my rows to be labeled as 'A' to 'H' and my columns as '1' to '12' in the 'By Position' view, i set 'Map Index to Characters' to 'true' for the 'Row' position factor.
Creation of the plate
To use my configuration of the position factors for the creation of the plate, I change to the 'Observations' view and I choose 'Assignments' > 'Position' > 'Row ? Column'. The possible selection 'Row ? Column' results from the chosen identifiers for the position factors. The table has 96 rows now with unique 'Row' - 'Column' combinations (A1 to H12).
In the 'By Position' view, the plate design is illustrated. For each well on the plate, I assign the 'Observation Group ID' and the 'Sequence Step'. (Multi-select is possible and useful here). This is done by selecting the cell(s) of the plate and then double-clicking the appropriate 'Value' field in the table below, where the correct ID and step can be chosen from a drop-down menu.
To switch between the assigned values of the different properties showable at the plate overview, the drop-down menu above the plate can be used. In the next picture, 'Sequence Step' is selected.
Input of the responses
The response values can be assigned in the same way as Observation Group IDs and Sequence Steps (multi-select might be questionable in this case), or via a data acquisition module. If a data acquisition module is used, it has to be made sure that the order of the imported responses matches the order of the responses of the data table in the 'Observations' view. In my example, I put in the responses manually.
Now the actual plate design is mapped to my QRA document and can always be retraced by using the 'By Position' view.