Features – Dose-Response Analysis Package

  • Interpolation of unknown analyte concentration using a calibration curve, e.g. ELISA or determination of residual protein
  • Regression models
    • Linear regression
    • 3,4, and 5-Parameter Logistic Fit
  • Calculate and compare multiple independent model fits, e.g. for assay optimization
  • Determination of curve characteristics: parameters, EC50, Ratio-of-Asymptotes or any effective concentration of interest
  • Spike-and-recovery analysis
  • Sample preparation
    • Multiple ways to define concentration sequence
    • Document whole preparation procedure, from source material to working sample
    • Define concentrations by stock solution, source material, or initial working concentration
  • Outlier Detection (4 methods)
    • Configurable outlier handling: replace, remove, mark for inspection
  • Response data processing
    • Response adjustment by fixed value or by control line
    • Response normalization by fixed value or by control line
    • Response averaging
    • Order and scope of processing steps is fully configurable
  • Test system
    • Assay and sample suitability
    • Hypothesis testing, tests on parameters, test on ANOVA results, tests on interpolation results
    • Configurable test severity
    • Precise control of test scope
  • Grouping: assign samples to groups
    • Document the factors your samples are grouped by
      • Overlay plots for each group
      • Group results: review results for all relevant sample at a glance