Dose-Response Analysis Package

Current version: 1.0, Release date: September 14, 2020

Abstract

The Dose-Response Analysis Package provides various biostatistical methods for immunoassays, ELISA, and more. It supports you in investigating equivalence for calibration curves, provides enhanced data processing, and options for subgroup analysis.

Interpolation on calibration curves

Plot the observation data of the Standard sample and the Test samples, calculate the standard curve, and determine the unknown concentration by interpolation on this curve.

The left graphic shows an interpolation plot for a group of Test samples, the right graphic an interpolation plot of a Test sample with response data adjustment by a fixed value of  1. Both plots were created using sample documents provided for the Dose-Response Analysis Package add-on in PLA 3.0.5. The plot on the left is using the '4PL calibration curve with report group' document, the plot on the right the 'Linear calibration curve (enhanced response data processing)' document.

Spike-and-recovery analysis

Spike your Test samples with different concentrations of your analyte to identify matrix effects or determine the precision of your assay within the assay range.

The graphic shows an interpolation plot of a Test sample with a medium spike, created using the 'Spike and recovery analysis' sample document provided for the Dose-Response Analysis Package add-on in PLA 3.0.5.

Linearity-of-dilution assessment

Determine the precision of your method at different levels of dilution.

The graphic shows an interpolation plot for Test samples assigned to the Medium-level sample report group, created using the 'Linearity of dilution assessment' sample document provided for the Dose-Response Analysis Package add-on in PLA 3.0.5.

Effective-concentration calculation

Calculate the effective concentration (for example EC50) of your sample in the concentrations you require by adding additional calculations.

The graphic shows a plot for concentration calculations for a Test sample, created using the 'Effective concentrations (ECx)' sample document provided for the Dose-Response Analysis Package add-on in PLA 3.0.5

Curve comparisons

Individually plot Test samples, and use the document dashboard or one of the document reports to compare the resulting curves. We support various analytical (regression) models.

The graphic shows a regression plot for a group of multi-dose samples, created using the 'Curve comparison' sample document provided for the Dose-Response Analysis Package add-on in PLA 3.0.5

 

Equivalence margin development

Use your historic assay runs to develop acceptance criteria. The Dose-Response Analysis Package provides test strategy development, visualizations, and simulations.

The graphic shows a plot depicting an equivalence test for parameter estimates, created using the 'Equivalence margin development and verification' sample document provided for the Dose-Response Analysis Package add-on in PLA 3.0.5

Guides & Tutorial videos

Do you want to learn how to work with this add-on? Find comprehensive descriptions of the concepts and document types in our guides and tutorial videos.

  • What's New Guide (PDF)
  • Getting Started Guide (PDF)