The Dichotomous Assay Package can be used to analyze assays based on binary responses (in contrast to quantitiative or continuous responses). For example, the physical reaction by cells or organisms to a substance can be classified as a 'yes' or 'no' response. In a dilution sequence of the substance, more cells or organisms typically show a reaction at higher doses. This leads to a probability distribution at every step. The Dichotomous assay document type provides you with tools to document, prepare, and analyze your assay data. Preserve assay information in the document and attach external files to the document to keep all relevant information in one place. Develop your analytical method by choosing between probit and logit models, and fine-tune statistical settings. Prove the validity of your assay with a large set of configurable tests on the calculated values. For example, you can specify margins of the values and which type of samples (reference, test, control) should be affected. After calculation, the Dashboard provides a first look at the results, including potency estimation for the test samples, and an overview of the regression and suitability tests. You can also generate reports which contain detailed information about the assay setup and analysis, such as the half-maximal effective concentration (EC50).