Spike your Test samples with different concentrations of your analyte to identify matrix effects or determine the precision of your assay within the assay range.

Examples of a Spike-and-recovery analysis with PLA 3.0

The following image from the 'Spike and recovery analysis' sample document shows results for Test sample TST17. The interpolation results are displayed in a data plot and as a table.

Please note: The response data of all Test samples in the sample document is adjusted by an unspiked Control sample.

The following image from the 'Spike and recovery analysis' sample document shows Test samples that have been spiked with a pure analyte to a concentration of 40 pg/ml. The response data of all Test samples in the sample document is adjusted by an unspiked Control sample. The interpolation results are displayed in a data plot and as a table.

Example report of a Spike-and-recovery analysis with PLA 3.0

  • Example report of a Spike-and-recovery analysis with PLA 3.0 839 KB
    This report shows how to use the Dose-Response Analysis Package for PLA 3.0 to perform spike and recovery analysis. It contains (artificial) observations for a standard sample, 21 test samples, and seven control samples. The standard is measured along a two-fold geometric sequence with seven steps where each measurement is taken in duplicate. Each test and control sample is measured in triplicates. For each sample matrix, three test samples represent the spiked samples, and one control sample represents the unspiked matrix. Spiked samples are assigned a concentration according to the spike, and recoveries are calculated and tested. Additionally, all test samples with the same spike concentration are collected in a report group, and a combined recovery for each group is calculated.