Spike your Test samples with different concentrations of your analyte to identify matrix effects or determine the precision of your assay within the assay range.
The following image from the 'Spike and recovery analysis' sample document shows Test samples that have been spiked with a pure analyte to a concentration of 40 pg/ml. The response data of all Test samples in the sample document is adjusted by an unspiked Control sample. The interpolation results are displayed in a data plot and as a table.