Dichotomous assay

quantal response assay, binary assay

A dichotomous assay (quantal response assay or binary assay) is a biological dilution assay based on a binary outcome. E.g. a number of specimens out of a total number of specimens shows a specified physiological response. The raw data of a dichotomous assay is a tupel of two integer numbers.

PLA 3.0 supports the biostatistical analysis of dichotomous assays with the probit or logit fit functions.

With PLA 3.0, you are able to calculate the potency of each standard/sample combination of your bioassay separately or simultaneously.


  • Dichotomous assay Report (Insulin) 114 KB
    Example taken from 'Statistical Method in Biological Assay' by D.J. Finney, 3rd Edition, 1978, Griffin. Data as given on page 376. Calculated potency and 95% confidence limits are in agreement with the computer-print on page 387.